Hi Alexis,

Thought the easiest way might be to record a quick video demo of this (can't do a voiceover right now - sitting in meetings). Short story is that it's still a little clunky and expects you to know a few things about general glycan structure, but it's pretty straightforward once you get the hang of it.

Video: https://drive.google.com/file/d/1U60eIAAwlDcOu4ajgkQ10p-bQ9qzIeE3/view?usp=sharing

Key points:

- the Asn amide should be in trans, and the sugar's H1 should also be trans to the amide hydrogen

- the "stem" is (Asn)-NAG-NAG-BMA, and (except in extremely rare cases) the sugars are arranged such that the ring oxygen alternates sides from one sugar to the next

Best,

Tristaan

On Thu, Nov 9, 2023 at 6:21 AM Alexis Rohou via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:

Hi there,

How would you recommend I go about building an N-linked glycan chain off of an ASN in an existing model using ChimeraX or ISOLDE? Is there documentation on this somewhere?

Cheers,
Alexis
_______________________________________________
ChimeraX-users mailing list -- chimerax-users@cgl.ucsf.edu
To unsubscribe send an email to chimerax-users-leave@cgl.ucsf.edu
Archives: https://mail.cgl.ucsf.edu/mailman/archives/list/chimerax-users@cgl.ucsf.edu/