Hi

I'm trying to model the N-terminus of mature canine Apolipoprotein A-I using Chimera. However the actual sequence in uniprot is not that of the mature protein. Here is the sequence:

MKAALLTLAVLFLTGSQARHFWQQDEPQSPWDRVKDLATVYVDAVKDSGRDYVAQFEASA
LGKQLNLKLLDNWDSLSSTVTKLREQIGPVTQEFWDNLEKETEVLRQEMSKDLEEVKQKV
QPYLDDFQKKWQEEVELYRQKVAPLGSELREGARQKLQELQEKLSPLAEELRDRARTHVD
ALRAQLAPYSDDLRERLAARLEALKEGGGASLAEYHARASEQLSALGEKARPALEDLRQG
LLPVLESFKVSLLAAIDEATKKLNAQ

The sequence of the mature form is:

DEPQSPWDRVKDLATVYVDAVKDSGRDYVAQFEASA
LGKQLNLKLLDNWDSLSSTVTKLREQIGPVTQEFWDNLEKETEVLRQEMSKDLEEVKQKV
QPYLDDFQKKWQEEVELYRQKVAPLGSELREGARQKLQELQEKLSPLAEELRDRARTHVD
ALRAQLAPYSDDLRERLAARLEALKEGGGASLAEYHARASEQLSALGEKARPALEDLRQG
LLPVLESFKVSLLAAIDEATKKLNAQ

According to how the sequence is labeled in uniprot the first aspartate residue of mature canine

Apolipoprotein A-I starts at position 25, and this what Chimera uses. However, for the mature

protein this should start at position 1. How can I change the numbering system so the first

aspartate residue starts at position 1?

I would be very glad for your advice.

Thank you.

Tiglath