Hi all,

I am following the coloring sequence by conservation tutorial but ran into some unexpected behavior. I used the the exact commands, but when I ran the "color byattribute seq_conservation" command, I got an error, "No known/registered attribute seq_conservation." The association was successful so I don't know why the seq_conservation attribute is not being set. See below for the log of my commands. 

I also tried using my own structure and msa but ran into the same "No known / registered attribute seq_conservation" issue, despite associating with 0 mismatches. 

Am I somehow missing something, or is something wrong? I am using a version of ChimeraX downloaded in November 2020, running on Windows 10. 


open 121p 
121p title:
Struktur und guanosintriphosphat-hydrolysemechanismus des C-terminal verkuerzten menschlichen krebsproteins P21-H-ras [more info...]

Chain information for 121p #1
Chain Description
A H-ras P21 protein
Non-standard residues in 121p #1
GCP — phosphomethylphosphonic acid guanylate ester
MG — magnesium ion
121p mmCIF Assemblies
1 author_defined_assembly

hide solvent
style ligand ball 
Changed 32 atom styles
open https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln
Summary of feedback from opening //www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln 
fetched from https
notes Fetching 121p-consurf(1).aln from https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln
Downloaded 121p-consurf.aln to C:\Users\selua\Downloads\121p-consurf(1).aln
Alignment identifier is 121p-consurf(1).aln
Associated 121p chain A to Input_pdb_SEQRES_A with 0 mismatches
Opened 150 sequences from 121p-consurf(1).aln
color byattribute seq_conservation 
No known/registered attribute seq_conservation

image.png

Thanks for any help,
Etowah