Hello

I have recently tried to use the bond rotation feature of Chimera X (Windows 10), and I have not been able to get it to work.

I have tried the Bond rotation button on the toolbar, and the torsion command to no avail.

The bond rotation button does not seem to do anything even when I click and drag with right click.

The Torsion command states that my bond “is part of a ring/cycle and cannot be rotated” or “To set torsion, middle two atoms (/A ARG 543 N C) must be bonded;they aren't”

I want to ask how do I use this function to rotate upon a simple amide bond.

I am not sure am I just selecting the wrong atoms/bonds or is the function just locked for this PDB.

The PDB file I am using is 7Y4P and I am trying to rotate about residues 543-544.

I have also tried to do it in normal Chimera’s rotation command with no results.

Thank you any advice would be welcome.

Regards,

Chun Po Fung