I did an initial port of Segger from Chimera to ChimeraX (also called Segment Map) but that code was written by Greg Pintilie and he has not worked the ChimeraX version so there is no new command to run it in a script.  It would probably not be too hard to write some Python to run it.  The Chimera script mentioned in the mailing list is attached to my message about it. 

That won't work in ChimeraX, but here I attach the equivalent for ChimeraX.

  Segger was developed to segment molecules in EM maps, so I have doubts that it will do much good for your light microscopy.  But you can decide that by running it by hand on some time steps and see if you like the result.

  Here is a different approach we used to quantify protrusions of crawling neutrophils in light sheet microscopy by placing markers that might be of interest.

  Tom