Thank you for your attention and suggestions. I have read the command, but when I attempted to display only the density maps for several amino acids, I was unable to make it happen. I earnestly look forward to your professional guidance and would be extremely grateful if you could spare the time to assist me. Thank you very much! ------------------ 原始邮件 ------------------ 发件人: "ChimeraX Users Help" <meng@cgl.ucsf.edu>; 发送时间: 2025年4月24日(星期四) 晚上11:58 收件人: "张馨丹 四川大学生物治疗国家重点实验室"<1025049892@qq.com>; 抄送: "chimerax-users"<chimerax-users@cgl.ucsf.edu>; 主题: Re: [chimerax-users] How can I overlay the 2Fo-Fc density map onto the amino acid residues I specified? Without knowing your exact data, I can only guess that maybe the "volume cover" command is what you need. see <https://rbvi.ucsf.edu/chimerax/docs/user/commands/volume.html#cover>; I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Resource for Biocomputing, Visualization, and Informatics Department of Pharmaceutical Chemistry University of California, San Francisco > On Apr 24, 2025, at 2:26 AM, 1025049892--- via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote: > > Every time I open the map file in ChimeraX, I find that it doesn't overlap with my PDB structure. Why is this the case, and how can I make them match? > > Thank you for your attention. I'm looking forward to your reply.