To run AlphaFold for more than one sequence you need the ChimeraX daily build (aka 1.4).  The error you got suggests you are using ChimeraX 1.3 which only handles a single sequence.

The Internal Server Error can be ignored -- it may be the UCSF servers are down, it is just trying to check what plugins are available.

Tom

On Mar 23, 2022, at 6:29 PM, Gawargi, Flobater I via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:

Hi, 
I am a new Chimerax user and I have encountered a problem that I hope you can help me fix. I installed 1.4 version and it shows "Startup Messages notes available bundle cache has not been initialized yet Updating list of available bundles failed: Internal Server Error” all I am trying to do is use alpha fold to predict structure of 2 proteins separated by comma. And it shows this error "Missing or invalid "sequences" argument: Sequences argument "cvfpfvflgkqyssctsdgrrdgrlwcattsnfdtdkkwgfcpdqgyslflvaahefghalgldhssvpealmyplysylegfplnkddidgiqylygrgskpdprppattttepqptapptmcptipptayptvgptvgptgapspgptsspspgptgapspgptapptagsseasteslspadnpcnvdvfdaiaeiqgalhffkdgwywkflnhrgsplqgpfltartw" is not a chain specifier, alignment id, UniProt id, or sequence characters” 
I am using an M1 MacBook, but I have also tried it on windows running windows 10 and got the same issue. I would appreciate your input to fix this issue. 

Thank you, 
Flobater Gawargi 







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