Hi Michel, I thought of a quick answer, though forgive me that I'm not Tristan. Have you tried first running the ChimeraX dssp command to assign the secondary structure, and next, choosing one of the secondary structure restraints buttons in the ISOLDE GUI's Restraints tab?


--
Dan Richman, PhD   (he/him/his)
Research Associate, James Berger Lab
Dept of Biophysics and Biophysical Chemistry
Johns Hopkins University School of Medicine
mobile: 201-669-0967
From: michel.thepaut--- via ChimeraX-users <chimerax-users@cgl.ucsf.edu>
Sent: Wednesday, February 25, 2026 6:15 AM
To: chimerax-users@cgl.ucsf.edu <chimerax-users@cgl.ucsf.edu>
Subject: [chimerax-users] Re: ISOLDE and secondary structure restraints
 

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Dear Tristan,
I have a similar question then I exhume that topic...

As I'm dealing with a ~1500 residues protein that I want to make dance in a density. Then as it is large I would like to use command lines to assign secondary structure to known helices and B-sheets instead of clicking on each residues to say this is an helix and this is a sheet.
Is there a way to say to Isolde  something like: 20 to 30 is an helix, 50 to 56 is a parallel sheet, ..., 1452 to 1460 is and helix. Please place your tensors and angles and lets dance?...

Thank you for your answer.
Michel
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