Hello!

I’ve been predicting structures recently using the AlphaFold tool through ChimeraX (which directs to the alphafold21_predict_colab.ipynb notebook), but I’ve noticed I get often quite different results when I run the same prediction through the AlphaFold.ipynb notebook directly in Colab. I was wondering if anybody could point me to the major differences between these two implementations? Are they searching different libraries to generate a multiple sequence alignment? Are they using different numbers of iterations or energy minimization strategies?

Thanks for any guidance,
Max

Max Coyle, PhD
Postdoctoral Fellow, Bellono Lab
Dept of Molecular and Cellular Biology
Harvard University
mcoyle@fas.harvard.edu