Dear All, I am sorry , i hit send before completely explain the issue. I think it is a formatting issue on the defattr input file, which i am not able to identify. I have attached the input file as attachment. Kindly review and suggest what could be done to fix the same. thanks again for your support., best amith ________________________________ From: ChimeraX-users <chimerax-users-bounces@cgl.ucsf.edu> on behalf of Rangarajan, Amith via ChimeraX-users <chimerax-users@cgl.ucsf.edu> Sent: Thursday, February 24, 2022 9:47 AM To: ChimeraX Users Help <ChimeraX-users@cgl.ucsf.edu> Subject: Re: [chimerax-users] correct format for attribute information file (.defattr) Hello everyone, I am trying to add mutation counts as a attribute and use it to generate a map of hotspots on the protein . I followed the format provided on chimerax documentation and i am getting this error : ZjQcmQRYFpfptBannerStart This Message Is From an External Sender This message came from outside your organization. ZjQcmQRYFpfptBannerEnd Hello everyone, I am trying to add mutation counts as a attribute and use it to generate a map of hotspots on the protein . I followed the format provided on chimerax documentation and i am getting this error : ZjQcmQRYFpfptBannerStart This Message Is From an External Sender This message came from outside your organization. ZjQcmQRYFpfptBannerEnd Hello everyone, I am trying to add mutation counts as a attribute and use it to generate a map of hotspots on the protein . I followed the format provided on chimerax documentation and i am getting this error : SyntaxError: Line 12 in KIT-mutations-count.defattr is either not of the form 'name: value' or is missing initial tab File "C:\Program Files\ChimeraX 1.3\bin\lib\site-packages\chimerax\std_commands\defattr.py", line 107, in defattr raise SyntaxError("Line %d in %s is either not of the form 'name: value'" i am on windows 11 using chimerax 1.3. thanks amith