Hi Shahid, (1) The “symmetry” option of the “volume” command only marks the specified map with the specified symmetry — it does not split or segment that map, so I don’t understand the series of actions you are trying to perform. The command also looks wrong. Is the map #1? (Does C33 = 33-fold cyclic symmetry even exist??) If the map is #1 and you wanted C33 symmetry, command syntax would be something like: volume #1 symmetry C33 axis z centerIndex 107.5 <http://rbvi.ucsf.edu/chimerax/docs/user/commands/volume.html#dimensions> (2) it depends what mean by “created segments” … how did you create them? If you used the Segment Map tool, it has its own File menu with options for saving: <http://rbvi.ucsf.edu/chimerax/docs/user/tools/segment.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Sep 11, 2019, at 5:24 AM, Shahid Khan <smkhan@lbl.gov> wrote:
Hello
I am having difficulty in manually imposing symmetry on a map (*.mrc file) and then extracting the unit cell for the subsequent fitting of PDB structures with fitmap.
(1) When I use the command "Volume symmetry #1, C33 axis z centerIndex 107.5"
I get the error message "No placements for #1" that I do not understand.
(2) I cannot figure out from the help documentation on how to save and display one of the created segments.
Any ideas? Thanks
Shahid