Dear colleagues, I have a cryo-EM map with different subunits and models for each unit. Is there a way to extract the cryo-EM density for each unit of this map? The units are pretty numerous😊 Thank you. Kind regards, Dmitry
Hi Dmitry, I did work on a similar multimeric complex and played with the segmentation tool, then grouping (or ungrouping) the monomers in Chimera (also Chimera X) helped and I was able to even distinguish between the spurious/dubious structures deposited on EMDB ( https://www.ebi.ac.uk/emdb/search/EcoR124%20Gao). Best, Pradeep On Thu, 16 Nov 2023 at 16:49, Dmitry Semchonok via ChimeraX-users < chimerax-users@cgl.ucsf.edu> wrote:
Dear colleagues,
I have a cryo-EM map with different subunits and models for each unit.
Is there a way to extract the cryo-EM density for each unit of this map?
The units are pretty numerous😊
Thank you.
Kind regards,
Dmitry _______________________________________________ ChimeraX-users mailing list -- chimerax-users@cgl.ucsf.edu To unsubscribe send an email to chimerax-users-leave@cgl.ucsf.edu Archives: https://mail.cgl.ucsf.edu/mailman/archives/list/chimerax-users@cgl.ucsf.edu/
Hi Dmitry, If you have an atomic model with the different subunits fit into the map then color it by chain and use the color zone tool to color the map to match nearby atoms, and then the Split Map button on the color zone tool (menu Tools / Volume Data / Color Zone). https://www.cgl.ucsf.edu/chimerax/docs/user/tools/colorzone.html Tom
On Nov 16, 2023, at 3:17 AM, Dmitry Semchonok via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
Dear colleagues,
I have a cryo-EM map with different subunits and models for each unit.
Is there a way to extract the cryo-EM density for each unit of this map?
The units are pretty numerous😊
Thank you.
Kind regards,
Dmitry
_______________________________________________ ChimeraX-users mailing list -- chimerax-users@cgl.ucsf.edu <mailto:chimerax-users@cgl.ucsf.edu> To unsubscribe send an email to chimerax-users-leave@cgl.ucsf.edu <mailto:chimerax-users-leave@cgl.ucsf.edu> Archives: https://mail.cgl.ucsf.edu/mailman/archives/list/chimerax-users@cgl.ucsf.edu/
participants (3)
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Dmitry Semchonok -
Pradeep HN -
Tom Goddard