Dear Bing Xu, The script file hb3.cxc that is linked to that example has all of the commands that are used to make it: <https://www.rbvi.ucsf.edu/chimerax/features.html#hbond-histogram> <https://www.rbvi.ucsf.edu/chimerax/feature_highlights/hb3.cxc> So I can't really get much more detailed than that. If you mean that the side chains are not displayed, make sure you use the "reveal t" option of the "hbonds" command as shown in the script. The command that shows the H-bond histogram is "crosslink" near the bottom of the script. To say anything more specific to your situation, I would need your exact data and the exact commands that you used. Best, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Jan 5, 2022, at 12:15 PM, Bing Xu via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:

Dear ChimeraX staff, I am sorry to bother you. I used ChimeraX to analyze the two protein complexes. I followed the guide for interactive H-Bond Histogram, but I still can not get the interaction amino acid of two proteins like the examples in ChimeraX Web

<image001.png> I am wondering whether you can provide me with more detailed guide for H-Bond histogram function. I do appreciate your help. Best, Bing Xu Research associate

Bing@mmrl.edu

Masonic Medical Research Institute Lab: 315-624-7481 / Fax: 315-735-5648 2150 Bleecker Street | Utica, NY 13501 www.mmrl.edu <image002.png>