Hi Yiannis, Without seeing your exact commands or files, it is hard to tell what went wrong. All I can say is: Get a reasonably new version of ChimeraX (in case you are looking at documentation newer than what you downloaded). All the maps must have the same grid size (numbers of points in each dimensions). <https://rbvi.ucsf.edu/chimerax/docs/user/trajectories.html> Use the "open" command with option "vseries true" ... that would indeed show just the first frame of the volume series, but you could play through it with the "vseries" command or slider interface. <https://rbvi.ucsf.edu/chimerax/docs/user/commands/open.html#vseries> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Sep 10, 2021, at 1:45 AM, Ioannis Skalidis via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:

Greetings to all!

I am trying to play a volume series data in ChimeraX (daily build). I open the multiple .mrc frames and they are automatically grouped, but then, when I use the vseries play command, I get an error message that I have not specified a volume series.

What am I doing wrong? Should I open the frames differently? I also tried to open them with the open command, a wildcard character for the frame names and vseries true modifier but even then, it only opens the first frame, which I found very strange as well. Could somebody offer some advice? It would be greatly appreciated!

Cheers, Yiannis.