Hi Pattana, That brain segmentation was not done in ChimeraX. It used custom machine learning plus 4000 hours of manual corrections by students in custom editing software. But for much simpler cases a few ChimeraX approaches could do. One is to place markers interactively with the marker mouse modes and then take all density within some distance with command volume zone. Can also take density near an approximately fit atomic model, but that probably won’t be useful for lower res EM. Another method is use the Volume Eraser tool to erase everything outside the region of interest. It lets you move a sphere around and erase with it. These ChimeraX methods are really simplistic and usually not sufficient. You would probably do better segmenting manually in IMOD. Tom

On May 13, 2020, at 12:38 PM, Pattana Jaroenlak (Michael) <p.jaroenlak@gmail.com> wrote:

 Hello,

I saw the twitter post from ChimeraX on segmentation of EM serial images (https://twitter.com/ucsfchimerax/status/1260425173238415360?s=12). It was very beautiful. I have serial section EM images. I am wondering that can I do manual segmentation on ChimeraX? Some cellular features on my images can not be picked up by thresholding or watersheding.

Thank you PJ

-- Pattana (Michael) Jaroenlak, PhD Bhabha & Ekiert Labs Skirball Institute of Biomolecular Medicine New York University School of Medicine Email: pattana.jaroenlak@nyulangone.org, p.jaroenlak@gmail.com _______________________________________________ ChimeraX-users mailing list ChimeraX-users@cgl.ucsf.edu Manage subscription: https://www.rbvi.ucsf.edu/mailman/listinfo/chimerax-users