Hi all I am currently working on a project, which needs to rebuild the statistics using modified protein structures, say, some other set of proteins. There is one quick question about interpreting the BBDep Library. What's the exact definition of the fourth column "N" in the library file format? I thought for each residue within some (phi,psi) angle pair bin, it is the total number of sidechains found in all of the 850 proteins. However, I checked ASN and GLN these two types of amino acids using "Chimera" to return the (phi,psi) dihedral angles for each ASN and GLN residue among 850 proteins with tens of exceptions which have no dihedral angles returned from Chimera(I just ignore them). Then, I count the number of sidechains for each (phi,psi) bin(10*10 degree). It turned out that this number is not consistent with the "N" value in BBDep. e.g. For ASN at bin (phi=-70,psi=-40), Chimera returns 372 sidechains in total from those 850 proteins, while BBDep says 2348. The difference is so large and very common over almost all the bins. I thought probably there might be some stupid errors in my understanding of the "N" value, but could you point that out for me? Or, did Dunbrack guys use another set of proteins other than those 850 ones? Best regards and thanks, Yang Lei Electrical & Computer Engineering University of Massachusetts, Amherst