Hi Milad, An easy way to make a fusion protein is to use ChimeraX and run AlphaFold to predict the structure. AlphaFold is not available in our old Chimera program -- no new features have gone into Chimera in over 5 years and many new capabilities are in ChimeraX. In ChimeraX, use menu Tools / Structure Prediction / AlphaFold, paste in your fusion protein sequence and press Predict. You may need to log into your Google account since the calculation is run on Google Colab servers. Calculation time is 5 minutes to a few hours depending on sequence length. Tom
On Aug 4, 2022, at 7:40 AM, Eric Pettersen via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:
Begin forwarded message:
From: Milad Mapar <milad.mapar@yahoo.com <mailto:milad.mapar@yahoo.com>> Subject: Making Fusion Proteins using Chimera Date: August 4, 2022 at 6:26:46 AM PDT To: "pett@cgl.ucsf.edu <mailto:pett@cgl.ucsf.edu>" <pett@cgl.ucsf.edu <mailto:pett@cgl.ucsf.edu>>
Dear Eric
Hello
With thanks to your support on Chimera, could you please help me to make a Fusion Protein using Chimera 1.16
Any links to tutorials or hints would be appreciated
Many thanks
Pharmaceutical Biotechnology PhD Student Milad
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