Hi Greg, Thank you for your reply. I do have the libOSMesa.so exist in our /usr/lib64/ directory. But it seems not the one ChimeraX is trying to import. The ldd result show as the following: ldd ./libexec/UCSF-ChimeraX/lib/python3.8/site-packages/osmesa/libOSMesa.so.8.0.0 ./libexec/UCSF-ChimeraX/lib/python3.8/site-packages/osmesa/libOSMesa.so.8.0.0: /lib64/libm.so.6: version `GLIBC_2.29' not found (required by ./libexec/UCSF-ChimeraX/lib/python3.8/site-packages/osmesa/libOSMesa.so.8.0.0) linux-vdso.so.1 (0x00007ffe625ef000) libz.so.1 => /lib64/libz.so.1 (0x00007fbcc8d47000) libpthread.so.0 => /lib64/libpthread.so.0 (0x00007fbcc8b27000) libdl.so.2 => /lib64/libdl.so.2 (0x00007fbcc8923000) libLLVM-10.so.1 => not found libstdc++.so.6 => /lib64/libstdc++.so.6 (0x00007fbcc858e000) libm.so.6 => /lib64/libm.so.6 (0x00007fbcc820c000) libc.so.6 => /lib64/libc.so.6 (0x00007fbcc7e4a000) libgcc_s.so.1 => /lib64/libgcc_s.so.1 (0x00007fbcc7c32000) /lib64/ld-linux-x86-64.so.2 (0x00007fbcc97eb000) On our centos 8 VM, it shows as: linux-vdso.so.1 => (0x00007ffebc3c3000) libz.so.1 => /lib64/libz.so.1 (0x00007f2fcf0c3000) libpthread.so.0 => /lib64/libpthread.so.0 (0x00007f2fceea6000) libdl.so.2 => /lib64/libdl.so.2 (0x00007f2fceca2000) libLLVM-3.9-mesa.so => /lib64/libLLVM-3.9-mesa.so (0x00007f2fccd71000) libstdc++.so.6 => /lib64/libstdc++.so.6 (0x00007f2fcca68000) libm.so.6 => /lib64/libm.so.6 (0x00007f2fcc766000) libc.so.6 => /lib64/libc.so.6 (0x00007f2fcc3a3000) libgcc_s.so.1 => /lib64/libgcc_s.so.1 (0x00007f2fcc18c000) /lib64/ld-linux-x86-64.so.2 (0x000055f2cf96c000) librt.so.1 => /lib64/../lib64/librt.so.1 (0x00007f2fcbf84000) So I feel like the ChimeraX mesa is imported but not the system one. Best, Zhe