Hi all, I am following the coloring sequence by conservation tutorial <https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/conservation-c...> but ran into some unexpected behavior. I used the the exact commands, but when I ran the "color byattribute seq_conservation" command, I got an error, "No known/registered attribute seq_conservation." The association was successful so I don't know why the seq_conservation attribute is not being set. See below for the log of my commands. I also tried using my own structure and msa but ran into the same "No known / registered attribute seq_conservation" issue, despite associating with 0 mismatches. Am I somehow missing something, or is something wrong? I am using a version of ChimeraX downloaded in November 2020, running on Windows 10.
*open 121p*
*121p title:*
Struktur und guanosintriphosphat-hydrolysemechanismus des C-terminal verkuerzten menschlichen krebsproteins P21-H-ras [more info...]
Chain information for 121p #1 Chain Description A H-ras P21 protein Non-standard residues in 121p #1 GCP — phosphomethylphosphonic acid guanylate ester MG — magnesium ion 121p mmCIF Assemblies 1 author_defined_assembly
*hide solvent* *style ligand ball*
Changed 32 atom styles
*open https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.a... <https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln>* Summary of feedback from opening // www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln
fetched from https
notes Fetching 121p-consurf(1).aln from https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.a... Downloaded 121p-consurf.aln to C:\Users\selua\Downloads\121p-consurf(1).aln Alignment identifier is 121p-consurf(1).aln Associated 121p chain A to Input_pdb_SEQRES_A with 0 mismatches Opened 150 sequences from 121p-consurf(1).aln *color byattribute seq_conservation*
No known/registered attribute seq_conservation
[image: image.png] Thanks for any help, Etowah
