Hi Jesse,
There is also a graphical user interface ChimeraX menu Tools / Volume Data / Measure Volume and Area that can measure the volume enclosed in STL surfaces. If the STL surface has holes, for instance at the top or bottom of the tomogram then this tool or the measure volume command will report that it has holes and compute the enclosed volume as if the hole were covered by a flat cap. If the surface has holes that are not flat in shape then it still covers the holes but the shape of the cap is irregular so the "enclosed volume" may be less meaningful.
Tom
On Dec 20, 2023, at 10:54 AM, Elaine Meng via ChimeraX-users chimerax-users@cgl.ucsf.edu wrote:
Hi Jesse, If the different components are different surface models in ChimeraX, you could use the command "measure volume," e.g. if the surface of the component of interest is model #3:
measure volume #3
...which would give cubic whatever distance units your data are in (angstroms, nanometers, ...)
See help for command options: https://rbvi.ucsf.edu/chimerax/docs/user/commands/measure.html#volume
I hope this helps, Elaine
Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Dec 20, 2023, at 7:29 AM, Jesse Hansen via ChimeraX-users chimerax-users@cgl.ucsf.edu wrote:
dear ChimeraX users,
We have a tomogram volume (.mrc) which we have segmented different components (stl files; surfaces). Is there any tool available to estimate the volume within each of the cellular components (within each surface)? Either some approximation of the cubic Angstrom/nanometers, or as a percentage of the total tomogram volume.
thanks in advance! best Jesse Hansen
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