Hi, Stéphane, The pixel size is just a value in the header of the MRC file, and you can change it with Relion: relion_image_handler --i input.mrc --o output.mrc --force_header_angpix [new_value] Best wishes, Vadim Kotov Postdoctoral fellow, Löw group EMBL Hamburg On 17.06.21 10:07, Stéphane ROCHE via ChimeraX-users wrote:
Dear Mr/Mrs,
I am new to ChimeraX (v 0.7) and I have a trouble when saving a map.
I am working with a .ccp4 map from an electron microscopy project. I would like to slightly change the pixel size that was inaccurately estimated during data processing and chimeraX seems to be the program for doing that.
-Opening the map is fine.
-Then I change the pixel voxelsize which is also fine.
-I then save the modified map (I use the .mrc format here given that I do not find a .ccp4 format).
-However if I then use the map with my usual softwares (phenix, coot) it looks like pure noise. But the map looks normal with some other softwares (e.g. pymol). I also have no problem usually with my .mrc maps with any software so I would assume the problem is with the .mrc map I created
Do you know what I could do to solve this problem ?
Thanks,
Stephane Roche
Institut de Biologie Intégrative de la Cellule
91198 Gif sur Yvette
France
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