displaying different segmentations together

dear all, I have segmented different parts of my tomogram using different software packages. One outputs an MRC file which I Can display as either volume or surface. The other is exported from Dragonfly and I am unable to figure out how to export MRC so I have tried STL and OBJ. STL/OBJ will display in ChimeraX, but then if I try to load in the other segmentation MRC then the STL/OBJ will disappear. I cannot view both simultaneously. If I hide the mrc and "view all" then I can view the object. Conversely, if I hide the object and "view all" I can see the MRC. Any help would be appreciated. Is there any way to convert obj/stl to an mrc? thanks! Jesse

Hi Jesse, An MRC file is 3D image data, in other words numeric values on a 3D grid. An STL or OBJ file is a surface (a set of triangles). So they are not the same kind of data. You can create create a mask (3D image data with all ones inside the surface) using the ChimeraX volume mask command https://www.cgl.ucsf.edu/chimerax/docs/user/commands/volume.html#mask The problem not being able to see both the MRC and the STL is probably because the sizes are enormously different. This would be a problem with the software that wrote the file. You can look at the sizes (physical extent) of the data files using the ChimeraX command "info bounds", you can see and set the MRC file voxel size with menu Tools / Volume Data / Map Coordinates, and you can scale the size of the surface with the "surface transform" command https://www.rbvi.ucsf.edu/chimerax/docs/user/commands/info.html#bounds https://www.cgl.ucsf.edu/chimerax/docs/user/commands/surface.html#transform Tom
On Jun 1, 2023, at 1:11 AM, Jesse Hansen via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
dear all,
I have segmented different parts of my tomogram using different software packages. One outputs an MRC file which I Can display as either volume or surface. The other is exported from Dragonfly and I am unable to figure out how to export MRC so I have tried STL and OBJ. STL/OBJ will display in ChimeraX, but then if I try to load in the other segmentation MRC then the STL/OBJ will disappear. I cannot view both simultaneously. If I hide the mrc and "view all" then I can view the object. Conversely, if I hide the object and "view all" I can see the MRC. Any help would be appreciated. Is there any way to convert obj/stl to an mrc?
thanks!
Jesse _______________________________________________ ChimeraX-users mailing list -- chimerax-users@cgl.ucsf.edu <mailto:chimerax-users@cgl.ucsf.edu> To unsubscribe send an email to chimerax-users-leave@cgl.ucsf.edu <mailto:chimerax-users-leave@cgl.ucsf.edu> Manage subscription:
participants (2)
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Jesse Hansen
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Tom Goddard