
Hello everyone, I have the results from some AlphaFold runs, is there a way to open them with ChimeraX, so I can see the plDDT, without needing to run Alphafold from within ChimeraX? Thank you and best regards

Hi Daniel, The pLDDT scores are saved in the B-factor column in the PDB files returned by AlphaFold. If you open them, you should be able to do "color bfactor palette alphafold range 0,100". Best, Tristan ________________________________ From: ChimeraX-users <chimerax-users-bounces@cgl.ucsf.edu> on behalf of Daniel Trejo Banos via ChimeraX-users <chimerax-users@cgl.ucsf.edu> Sent: 10 February 2022 10:46 To: chimerax-users@cgl.ucsf.edu <chimerax-users@cgl.ucsf.edu> Subject: [chimerax-users] Loading AlphaFold output Hello everyone, I have the results from some AlphaFold runs, is there a way to open them with ChimeraX, so I can see the plDDT, without needing to run Alphafold from within ChimeraX? Thank you and best regards

Right, and you can even omit the "range" part because the color-value mapping is included in the palette, as described here: <https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html#coloring> color bfactor palette alphafold You could use a different palette in the color command, if you like. In case you didn't know already: ChimeraX also searches/fetches from the AlphaFold Database of already-made models and applies this coloring. Just an alternative if you were going to that database, downloading files yourself, then opening them in ChimeraX. The search/fetch can be done with either the AlphaFold tool or the alphafold command. <https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html> <https://rbvi.ucsf.edu/chimerax/docs/user/commands/alphafold.html> Best, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Feb 10, 2022, at 3:34 AM, Tristan Croll via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
Hi Daniel,
The pLDDT scores are saved in the B-factor column in the PDB files returned by AlphaFold. If you open them, you should be able to do "color bfactor palette alphafold range 0,100".
Best,
Tristan From: ChimeraX-users <chimerax-users-bounces@cgl.ucsf.edu> on behalf of Daniel Trejo Banos via ChimeraX-users <chimerax-users@cgl.ucsf.edu> Sent: 10 February 2022 10:46 To: chimerax-users@cgl.ucsf.edu <chimerax-users@cgl.ucsf.edu> Subject: [chimerax-users] Loading AlphaFold output
Hello everyone,
I have the results from some AlphaFold runs, is there a way to open them with ChimeraX, so I can see the plDDT, without needing to run Alphafold from within ChimeraX?
Thank you and best regards
participants (3)
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Daniel Trejo Banos
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Elaine Meng
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Tristan Croll