Re: [Chimera-users] Joining models question

Thanks Elaine. I was omitting the .A. Since they only had one chain I didn't think it was required. But it's working now. Best, Kyle Dr. Kyle J. Lauersen Algae Biotechnology & Bioenergy Center for Biotechnology Bielefeld University p: +49 (0) 521 106 12261 e: kyle.lauersen@uni-bielefeld.de

On 29.01.2016, at 20:43, Elaine Meng wrote:

Hi Kyle, The following works for me:

I opened YFP and PcPs in that order, then:

commands: ~ribbon disp @n,ca,c sel #1:559.a@C #0:2.a@N

menu: Tools….Structure Editing… Build Structure, change to Join Models section of the resulting dialog.

Then I chose the “C-N peptide bond” option and clicked Apply, and the join appears to take place successfully.

However, I see your point that something weird is going on: I repeated the process several times, and sometimes (but not always!!) if I didn’t change from ribbon to atom display, the Apply button was grayed out. We should look into why that is happening.

I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco

...

On Jan 29, 2016, at 5:33 AM, Kyle J. Lauersen wrote:

Hello,

I am trying to join the two models attached to this sequence.

I have used the sequence selection code in atom specifier: #1:559.a@C #0:2.a@N

Where model #0 is YFP and #1 is PcPs.

When I go to join models, the Apply button is not accessible.

Can you indicate if I am doing something incorrect in the selection of the C and N atoms?

Thank you for your time.

Kyle

Dr. Kyle J. Lauersen Algae Biotechnology & Bioenergy Center for Biotechnology Bielefeld University p: +49 (0) 521 106 12289 e: kyle.lauersen@uni-bielefeld.de

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