Dear Dr, I forgot to add the comment that the protein forms dimer on the SDS-PAGE. “Dimer Formation of a Cell-Cell Adhesion Protein, gp64 of the Cellular Slime Mold, Polysphondylium pallidum” Plans and Cell Physiology, Vol. 36, March 1996, pages 135-139 Hiroshi
2026/07/05 14:29、落合廣 <ochiai3@icloud.com>のメール:
Dear DR. Thank you for using chimeraX. I am very impressed with the development of protein assembly analysis technology. I am an elderly (88-year-old) biochemist. I have a question. ○1. Even if dimer formation is analyzed by AlphaFold 3, dimers with interfaces are not recognized at all (AlphaFold has a list of 19(?) analyzed). Why? ○2. Even though dimer formation (54 or 132 interface residues) can be seen by chimeraX, when looking at ipTM with AlphaFold, a number such as 0.19 is not recognized as a dimer. Do you think that this is because AlphaFold does not take into account that proteins such as GPI membrane proteins are kept in anti-parallel, do you have an opinion? With best Regards
Hiroshi Ochiai Research Institute for Electronic Sciences, Hokkaido University, Sapporo Japan Emeritus Professor