July 2018 - Chimera-users - RBVI Mailing Lists

Surface vertex density
by Tom Goddard 31 Jul '18

31 Jul '18

For a density map you can control the number of surface triangles and vertices by choosing the subsampling “step” size in the Volume Viewer dialog. Step 2 will use every other grid point along x,y,z axes and result in a coarser mesh. For molecular surfaces computed from atoms, change the vertex density as described here http://plato.cgl.ucsf.edu/pipermail/chimera-users/2011-January/005889.html <http://plato.cgl.ucsf.edu/pipermail/chimera-users/2011-January/005889.html> Tom > On Jul 31, 2018, at 5:53 AM, ⁨עמיחי הולצר⁩ wrote: > > And another question - there is "Surface and Mesh options" under the "Features" tab, so one can control the smoothing of the surface, thickness, etc. How can I control the number of nodes of the surface? I mean, I want the mesh not to be so dense. Is it possible? > > Thanks again! > > 2018-07-31 13:38 GMT+03:00 עמיחי הולצר : > When I save it as OBJ file, how can I watch it in chimera again as a molecule with "atoms" and "bonds" (like CONECT line in pdb file)? > I see that OBJ file look like a xml file, and contains coordinates and edges, but does it reflect the mesh? > > Thanks, > Amichai > > 2018-07-30 19:37 GMT+03:00 Tom Goddard : > Hi Amichai, > > Chimera computes density map isosurfaces using the marching cubes algorithm. It can be saved in various file formats for example OBJ, others are described with the export command > > https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/export.html <https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/export.html> > > Tom > > >> On Jul 29, 2018, at 3:06 AM, ⁨עמיחי הולצר⁩wrote: >> >> Hi Chimera support, >> >> I have 2 questions: >> >> 1. How does Chimera calculate the mesh from the density map? >> >> 2. How can I save the mesh as a file? >> >> Thanks a lot, >> Amichai Holzer >> Huji >> _______________________________________________ >> Chimera-dev mailing list >> Chimera-dev(a)cgl.ucsf.edu <mailto:Chimera-dev@cgl.ucsf.edu> >> http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-dev <http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-dev> > >

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Re: [Chimera-users] [chimera-dev] Save density map as a mesh
by Tom Goddard 31 Jul '18

31 Jul '18

If you want to save atoms, bonds and surfaces and load them into Chimera later, then save a session, menu File / Save Session As…. Tom > On Jul 31, 2018, at 3:38 AM, ⁨עמיחי הולצר⁩ <⁨amiholz(a)gmail.xn--com-yx0a> wrote: > > When I save it as OBJ file, how can I watch it in chimera again as a molecule with "atoms" and "bonds" (like CONECT line in pdb file)? > I see that OBJ file look like a xml file, and contains coordinates and edges, but does it reflect the mesh? > > Thanks, > Amichai > > 2018-07-30 19:37 GMT+03:00 Tom Goddard <goddard(a)sonic.net <mailto:goddard@sonic.net>>: > Hi Amichai, > > Chimera computes density map isosurfaces using the marching cubes algorithm. It can be saved in various file formats for example OBJ, others are described with the export command > > https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/export.html <https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/export.html> > > Tom > > >> On Jul 29, 2018, at 3:06 AM, ⁨עמיחי הולצר⁩ <⁨amiholz(a)gmail.com <mailto:amiholz@gmail.com>⁩> wrote: >> >> Hi Chimera support, >> >> I have 2 questions: >> >> 1. How does Chimera calculate the mesh from the density map? >> >> 2. How can I save the mesh as a file? >> >> Thanks a lot, >> Amichai Holzer >> Huji >> _______________________________________________ >> Chimera-dev mailing list >> Chimera-dev(a)cgl.ucsf.edu <mailto:Chimera-dev@cgl.ucsf.edu> >> http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-dev <http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-dev> > >

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display floating XYZ axes
by Pranav Shah 26 Jul '18

26 Jul '18

Dear Chimera Users and Devs, Is there a possibility to display the XYZ axes *a la* coot in chimera, so that one can keep a track of the direction of the views in the window? Cheers, Pranav -- Pranav Shah Postdoctoral Research Fellow. Hogle Lab Harvard Medical School 240 Longwood Avenue Boston, MA 02115 (617) 432-4360 (fax) (617) 432-3839 (lab)

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(no subject)
by Yanhe Zhao 26 Jul '18

26 Jul '18

Hi, This is Yanhe. I want to make movies of side-by side rotaing structures, I set "center of rotation method" to "independent", when I try to save the scenes, chimera give error report as attached file shown. what should I do now? I really appreciate it if you can do me the favor. Best, Yanhe

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Removing structure segment and connecting the edges with dashed line
by Ahmad Khalifa 26 Jul '18

26 Jul '18

Hello, Selecting a segment of a structure and deleting it does remove that segment, but the ends are not connected. How to do the same and make the edges connected with a dashed line? Best regards.

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Problem with minimizing structure
by Renee Arias 26 Jul '18

26 Jul '18

Hello all, I am having a problem with the minimizing structure function in Chimera. I am just trying to change a single residue on a very small protein (88 residues), but I keep getting the error: No MMTK name for atom "H1" in standard residue "GLN". Previous message boards seem to just point to bug fixes in Chimera, but I am running the newest version available for Windows 10. I would appreciate any help! Thanks so much, Renee

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Click-and-drag selection still not working for chimera in Mac OSX....any plans for improvement
by Carmen Bayly 26 Jul '18

26 Jul '18

Hello Chimera users, I'm working with Chimera on an OSX 10.12.2 (intel core processor). I noticed that the click-and-drag selection function doesn't work, and saw a bug report from 2015 explaining it was because of the intel processor. However, it's 2018 now - does anyone know any fixes besides the wire display style? Thanks, Carmen

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action of labels
by Yanhe Zhao 25 Jul '18

25 Jul '18

To whom may concern, This is Yanhe Zhao, from ut southwestern medical center. After I add the 2D labels, i know how to move them, but could I rotate them? I really appreciate if someone can do me the favor. Best, Yanhe

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Aligning structure based on a specific secondary structure
by Ahmad Khalifa 25 Jul '18

25 Jul '18

Hello, All the options I see in Matchmaker is based on aligning whole chains. Is it possible to align structures based on a specific secondary structure, like a specific alpha helix for example? How to do so? Thanks in advance.

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How to place two identical proteins "near" each other
by Marla DeKlotz 24 Jul '18

24 Jul '18

I am trying to prepare a pdb to use in Rosetta docking. I need to place two copies of the same protein "near" each other, in roughly the alignment they should have after they dock together. Chimera seems to always overlay the two copies and I can't figure out a way to separate them and move/rotate each one independently. I don't want a tile view, I need them to have a real relationship in space so Rosetta knows where to "aim" its docking protocol. Thanks, Marla

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