- Chimera-users - RBVI Mailing Lists

calculating dipole moment of a protein in pdb
by Pande, Ajay K 25 Nov '15

25 Nov '15

Could you please tell me how to calculate the dipole moment of a protein in Chimera, given its pdb file? Thanks Ajay

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on ligand
by Smith Liu 25 Nov '15

25 Nov '15

Dear All, When we display a protein-ligand complex by chimera, the sidechains of residues around the ligand will be displayed. Will you please tell me by which criteria chimera select the residues arond the ligand for display? And how to change this criteria in the command input format? SMith

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feature request - add "save relative to model" as optional default?
by Oliver Clarke 25 Nov '15

25 Nov '15

Hi, Nitpick-y request for a future version but one that would make life easier... in the “Save PDB" dialog, would it be possible (when the option is present) to default (perhaps optionally) to having the “Save relative to model” box checked (and have the selected model default to the starting model)? I often save a manipulated pdb, or one that has been rigid-body fit to a density map, then open it up in coot or another program and realize that the map and model are no longer aligned because I forgot to check this box, and I can’t think of many cases when I would deliberately uncheck it. Cheers, Oliver.

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Re: [Chimera-users] scale problem
by Yaowang Li 24 Nov '15

24 Nov '15

Dear all, I faced a scale problem when I was trying to fit the density map. A density map was reconstructed from a set of projections which their magnitude are 1.055A/pixel, with a reference model that is 1.055 A/voxel and 5 A in resolution. However the pdb file cannot fit into the map because the map is too much small. So I have to change the voxel size to double and then can be fitted. Here I changed the voxel size, I think it changed the scale for the density. So i just worried about the fitting result. thanks On Sun, Nov 22, 2015 at 10:07 AM, Yaowang Li <liyaowang2911(a)gmail.com> wrote: > Dear all, > > > I faced a scale problem when I was trying to fit the density map. > > A density map was reconstructed from a set of projections which their > magnitude are 1.055A/pixel, with a reference model that is 1.055 A/voxel > and 5 A in resolution. However the pdb file cannot fit into the map > because the map is too much small. So I have to change the voxel size to > double and then can be fitted. > > Here I changed the voxel size, I think it changed the scale for the > density. So i just worried about the fitting result. > > thanks > > yaowang > > > > >

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Re: [Chimera-users] Calculation of the average isoelectric point of a proteins solvation shell
by Elaine Meng 23 Nov '15

23 Nov '15

> On Nov 21, 2015, at 11:54 AM, Csaba Nagy <csaba.nagy(a)utu.fi> wrote: > > Dear Elaine, > > thank you for your kind reply, and for the instructions regarding the surface charge of the protein. I just realized, that I phrased my question wrong, actually I wanted to calculate the average isoelectric constant of the proteins surface-amino acids in order to apply the most optimal buffer system to solubilize my protein. > Thank you again. > > Best wishes, > Csaba > Dear Csaba, Sorry, Chimera does not calculate isoelectric points or constants, it only estimates charge in aqueous solution near neutral pH. You would need to find some other program to do what you want. It is best to send Chimera questions to the chimera-users(a)cgl.ucsf.edu list, CC’d here. Apologies if you got this message more than once, I was having trouble sending the e-mail. Best, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco

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How to insert a morph into a movie command file?
by Christina Bourne 23 Nov '15

23 Nov '15

Dear all, Is there a straightforward way to insert a morph within a larger movie? We are working from command scripts but see that the command "morph" is not recognized.Any help is appreciated,Christina

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Dock prep with multiple structures - endless loop?
by Oliver Clarke 20 Nov '15

20 Nov '15

Hi, Would it be possible to add a progress bar (with ETA) for Dock prep at some point? I ran it overnight to fill partial sidechains on six structures (each ~6000aas), and it still isn’t finished, but I can’t tell whether it is just taking a really long time or whether something has gone wrong. The status bar is still updating with “Matching donors”, “finding acceptors” and “building search tree” messages, but it seems to be giving these messages multiple times for each structure - is that normal? Cheers, Oliver.

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Inter-molecular H-bonds between water molecules not displayed in per-frame script
by George Green 20 Nov '15

20 Nov '15

Hi, I'm trying to get a per-frame script going which shows the inter-molecular H-bonds within a protein binding site. It is working in that all H-bonds show apart from water-water h-bonds. Is there any fix for this at all? My code is below: Water molecules are residues :159-162 I would be very grateful for any assistance. :) sg ################################################ #################### # per-frame H-bond repr bs :158 color hot pink :158 color byhet :158 # disp :60,37,85,112,109,35,158,84,60,40,38,114,69,90,58,61-68,56,21,45,52,101,15,92,40,105,116,24,42,54,40,24,10,97,120,159,160,161,162 disp :WAT # select :1-162; hbonds intramodel true intermodel true line 5 color gold ; ~select select :90 ################################################

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Calculation of the average isoelectric point of a proteins solvation shell
by Csaba Nagy 20 Nov '15

20 Nov '15

Dear Chimera Developers and -Users, I am struggling with the overexpression and purification of a cytoplasmic protein that looks to be soluble only in a very narrow pH range. I know the average isoelectric point of the whole protein, but there would be much more informative, to know what is the average isoelectric point of the proteins solvation shell, I think. Therefore I modeled the 3D structure of the protein and I tried several other visualization programs to calculate this pI value, but I was not successful. Could you tell me please, if there is any option to make this estimation in Chimera? Thank you very much in advance! Best regards, Csaba Nagy ------------------------------- Csaba I. Nagy, PhD Postdoctoral Researcher, PharmaCity, Itäinen Pitkäkatu 4 C, 6th floor, room 6049 Molecular Plant Biology University of Turku FI-20014 TURKU, Finland Phone +358 2 333 7914 Fax +358 2 333 8075 Email csaba.nagy(a)utu.fi --------------------------------

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command line issues
by liz kellogg 19 Nov '15

19 Nov '15

Hi, I've been using chimera quite happily for the past couple of years. I especially like the command line, as it makes operations much faster to complete (once you know the commands!) I've been using the command-line, in chimera, on my mac (osx yosemite version 10.10.3) for a while now, but just today my command-line would not display the text I'm entering into the command-line bar. This is odd, I've never seen this before, and I don't know what the problem is due to. I tried restarting chimera, I tried restarting my computer, and I tried re-installing chimera, but the command-line prompt is the same.. it doesn't display the text as I'm entering it.. though if I type a command, it does execute it and it shows up in the command history.. so it looks like a problem in displaying text as it's being entered. Has anyone else run into this problem? And how do you fix it? I'd be so much happier if I could see what I'm typing! thanks Liz

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