- Chimera-users - RBVI Mailing Lists

Stuck in adding residue in the C-terminal of a segment
by Prathit Chatterjee 29 Jun '21

29 Jun '21

Dear Experts, This is regarding adding an ALA residue in the C-terminal of a specific protein domain, with CHIMERA. I have a protein-complex with multiple domains and chains. I want to add an ALA residue (101-th position) in the chain D, after the 100th residue which is PRO. I followed the tutorial and have given the following command in command line: addaa ala,101 :100.d The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D. Request you to kindly let me know what the possible solution could be, so that I add an ALA residue in the C-terminal of Chain D. Thank you in advance, Regards, Prathit Chatterjee

2 4

how to know the net charge of my system
by Sara Luz Gomez Maya 29 Jun '21

29 Jun '21

Dear all, I'd like to know if there is a way to know the net charge of a system when opening the pdb file with Chimera. If so, is it possible to do it by means of the command line? Thanks in advance, Sara

2 2

same chain id while saving large coordinate files
by manikandank 27 Jun '21

27 Jun '21

Hi Chimera-users, Chimera 1.15 (build 42258) puts same chain id for all protein chains present in the pdb during 'save pdb/write pdb' . I am using it in linux Ubuntu. Please help. thank you with kind regards Mani.

2 4

Modeller..Waiting for the Opal server
by Hernando J Sosa 26 Jun '21

26 Jun '21

Dear Chimera, Today when trying to use Modeller through the Chimera interface (via web service) the program either stays forever in Waiting for the Opal server or produces a whole bunch of errors. Trying to run Modeller through the local installation also results in errors. Not sure if the problem is with Chimera or Modeller but one day ago Modeller&Chimera were working fine with the same input files that now are causing the errors (???). Thanks Best Hernando

2 2

multi align viewer
by Jacqueline Vitali 25 Jun '21

25 Jun '21

Hi Elaine, When you do match-->align in chimera, you create the MAV. It has a coral region for the fully populated columns. If you change the order of the sequences, it automatically disappears. Is there a way to bring this region back? Thank you. Jackie Vitali Cleveland State University

2 4

multialign viewer
by Jacqueline Vitali 23 Jun '21

23 Jun '21

Hi Elaine, When you do match-->align in chimera, you create the MAV. It has a coral region for the fully populated columns. If you change the order of the sequences, it automatically disappears. Is there a way to bring this region back? Thank you. Jackie Vitali Cleveland State University

1 0

Regarding problem 'During selection from the graphics window (picking)'
by Dr. Abhay Nanda Srivastava 20 Jun '21

20 Jun '21

Dear Team Chimera, I am using Chimera software in Windows 7, 32 bit computer for viewing Swiss dock prediction results. But, when I try to select the residue or atom in model by using mouse and keyboard button (Ctrl+button 1), no selection is displayed, even hand like cursor seen on the graphics, I am also not able to see the residue or atoms labeling by putting cursor on model. For my confusion removal, I have downloaded and installed the software on another computer of Windows 7, 64 bit. But here also, the same problem persists. Please suggest to me, how to get rid of this problem? Looking forward to hearing from you. Thank You. *Regards,* *Dr. Abhay N. Srivastava* * M. Sc. (Chem.), Ph. D. Head & Asst. Professor,* * Department of Chemistry,* * NC-BRA Bihar University,* * Muzaffarpur-India.* * Contact: +91-9990576112*

2 1

Question regarding the chimera Transform Coordinates feature
by Ritwik Jain 19 Jun '21

19 Jun '21

Respected developers of chimera, I am currently doing a research project related to Cryo-EM map alignment in which I need to use Chimera. Chimera has a feature which transforms the coordinates of a molecule (Img1.jpg) . That feature has an option to move atoms instead of coordinate axes (Img2.jpg). If I uncheck that box and transform, the model just reverts back to its original position after saving and reopening. I was wondering if there was a command line command for the " move atoms instead of coordinate axes " option. I have tried all the move commands ( https://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/midas/move.html) and they don't seem to work. I expect your reply soon. Sincerely, Ritwik Jain

2 3

chimera installation problem
by Monojit roy 18 Jun '21

18 Jun '21

sir, please help

2 1

AddH on N atom in N-terminal
by 陈恩博 17 Jun '21

17 Jun '21

Hello. When I use addH to add H atoms on N atom in N-terminal of Peptide chain, I find 3 H were added on this N. Why the addH result is C-NH3 and not C-NH2? Thanks!

2 2