November 2025 - ChimeraX-users - RBVI Mailing Lists

Colouring a morph anim
by Pranav Shah 17 Nov '25

17 Nov '25

Hi Team, I am seeking advice on the most effective way to visualize a morphing trajectory within ChimeraX. Specifically, I have a multi-chain model transitioning (morphing) from its starting state to its final state, and I would like to know if there is a method in ChimeraX to apply a color gradient along this trajectory. Additionally, I would greatly appreciate guidance on how to color specific atoms, such as Cα atoms, based on their overall displacement. Ideally, the color intensity (hue) would increase in proportion to the magnitude of the displacement. Best regards, Pranav Best, Pranav -- Pranav Shah Postdoctoral Research Fellow. Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK

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Converting .stl to .mrc or .pdb
by mitchell.gulkis＠ufl.edu 17 Nov '25

17 Nov '25

Hello everyone, For instructional purposes, I am trying to generate simulated cryo-EM micrographs from a .stl file, in this case Mickey Mouse. This way, students can hold the 3D printed Mickey up to the micrograph to better understand projection images and angular assignment. I am able to open the .stl file in ChimeraX, but I can not figure out how to convert it to a format that is accepted by micrograph simulation programs, either .mrc or .pdb. Has anyone else figured out how to do this? Thanks! Sincerely, Mitch

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On the web Casino wars 101 The way to Take full advantage of The Payout
by pachat71＠teleworm.us 16 Nov '25

16 Nov '25

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PyPI Chimerax Package for Windows
by Berenice E Cowfer Chavez 14 Nov '25

14 Nov '25

Hello, Do you have a PyPI ChimeraX package for windows? I can online find a .whl file for macOS. Sincerely, Berenice E. Cowfer Chavez, M.S. Lab Manager, Profs. Johnson and Shoulders Labs Massachusetts Institute of Technology

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A fixed clipping plane?
by jon_choy＠g.harvard.edu 14 Nov '25

14 Nov '25

Hi all, Prior to this I was an avid IMOD user. There I was able to set a clipping plane and move/rotate the model such that the model clipping plan would NOT clip any other parts of the model. Essentially i want to create a clipping plane, set it and then transform the model such that no other parts of the model will be clipped. Is this possible? I could not figure it out for the life of me. It's pretty simple in IMOD, just not sure if it's possible in ChimeraX. Would love to hear what you guys think. -Jon

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color of capped surface
by stokes＠nyu.edu 12 Nov '25

12 Nov '25

I am having trouble with the surface color and specifically of capped surfaces. To start, surfaces of individual chains are given a single color, despite individual domains having been assigned a variety of colors. I can change the colors of the surface for individual domains subsequently, so that is not so bad. However, the color of the capped surface remains the initial color adopted when the surface was created. When I change colors of the individual chains, or domains, the surface color changes, but not the color of the capped/clipped surface. If I change the color of the entire model, then the surface cap changes. But it does not respond to subsequent changes of individual chains and domains. Hiding/showing the surface does not solve the problem (though I have a dim memory of this working in the past - maybe that was the old chimera). I am only using near/far clipping planes - not front/back. ChimeraX 1.10.1 on Ubuntu and 1.8 on Windows behave the same. Thanks in advance for any insight, David

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Blurry Volume Rendering in ChimeraX ≥1.7 on macOS (HiDPI Regression Affecting ArtiaX)
by Yaroslav Tsytsyura 11 Nov '25

11 Nov '25

A little comment to the message: The following text was generated with help of an AI, because I’ve got frustrated trying to solve the issue myself and ran out of strength; I apologise for that. Yet it describes the problem well: Dear ChimeraX/ArtiaX developers, I would like to report an issue with volume rendering quality on macOS that appears to have been introduced in ChimeraX versions 1.7 and later, and that affects ArtiaX-based tomography workflows. System details macOS 26.0.1 on Apple Silicon (M2 Pro; RAM 16GB) ChimeraX 1.6.1 (works correctly) ChimeraX 1.7–1.10.1 (affected) ArtiaX 0.3 (works correctly) ArtiaX 0.6 (affected) Native ARM64 ChimeraX binary used (not via Rosetta) Tested both on the built-in display and external 4K monitor Issue summary When displaying reconstructed tomograms (binned 4x to pixel value 10A) in ChimeraX 1.7 and newer, the volume appears blurry, pixelated, and significantly lower in resolution compared to Fiji or ChimeraX 1.6.1. The same tomogram, same hardware, same display settings, and same scaling factors show correct high-quality sampling in ChimeraX 1.6.1 + ArtiaX 0.3. In contrast, ChimeraX ≥1.7 + ArtiaX ≥0.6 consistently produce a downsampled-looking rendering. Troubleshooting performed To rule out common macOS/Qt/HiDPI issues, I have tested: Forcing HiDPI mode (QT_ENABLE_HIGHDPI_SCALING, QT_SCALE_FACTOR, etc.) Launching via arch -arm64 Removing and reinstalling ChimeraX completely (including preferences) Disabling external 4K monitor scaling Resetting ChimeraX user settings None of these affected the outcome. Only reverting ChimeraX to version 1.6.1 restores correct rendering quality. Behavior differences observed In ChimeraX 1.6, tomograms appear crisp and match Fiji’s display quality. In ChimeraX 1.7–1.10.1, the same tomograms appear visibly blurred and pixelated. The command graphics quality high or similar parameters appear unsupported or ineffective in newer versions. ArtiaX toolbar loads but seems to use lower-resolution textures for slice volumes. This strongly suggests a rendering pipeline change around the transition from ChimeraX 1.6.1 → 1.7 that affects ArtiaX’s volume display on macOS (possibly due to Qt scaling, offscreen framebuffer size, or texture sampling changes). Request Could you please check whether: ArtiaX 0.6 is triggering half-resolution internal textures on macOS? The new rendering pipeline introduced in ChimeraX ≥1.7 affects HiDPI texture sampling? There is a fallback path on macOS ARM that reduces framebuffer resolution? Any configuration options can restore full-resolution sampling? To illustrate the difference between the two options (ChimeraX 1.6 vs 1.10.1) I have included the snapshots from two different M2 MacBooks running two different versions of ChimeraX/ArtiaX combinations; the tomogram file, tomogram optical slice position are the same, the screen magnification and the regions displayed are very similar. Thank you very much for your work and for looking into this. Best regards, Yaroslav ------------------------------------------- Dr. Yaroslav Tsytsyura Cellular Biophysics Institute of Medical Physics and Biophysics University of Muenster Robert-Koch Str., 31 48149, Muenster GERMANY phone: +49 251 8351 010 fax: +49 251 8355 121 Mobile: +49 157 895 47789 web-page: https://www.medizin.uni-muenster.de/impb/das-institut/elektronenmikroskopie…

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Offscreen/headless render acceleration
by Roden Deng Luo 07 Nov '25

07 Nov '25

Hi, In https://www.cgl.ucsf.edu/chimerax/docs/user/options.html, under `--offscreen`, it says "It cannot use a graphics card or hardware-accelerated rendering because OpenGL does not support rendering without a window." I wonder if there are any possible ways to utilize graphics cards in the headless mode, or if you have any best practices for saving pictures/movies for hundreds to thousands of structures, with or without a GUI. Thanks, Roden -- This message and its contents, including attachments are intended solely for the original recipient. If you are not the intended recipient or have received this message in error, please notify me immediately and delete this message from your computer system. Any unauthorized use or distribution is prohibited. Please consider the environment before printing this email.

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front-back shadow
by Citterico, Matteo 07 Nov '25

07 Nov '25

Hello, I’m having trouble changing the depth cue start and end settings in ChimeraX 1.8. I’ve tried several commands, but none of them seem to work. Could you please help me with this? Thank you in advance, Matteo

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Recent spam
by Eric Pettersen 06 Nov '25

06 Nov '25

It seems that the era of AI bots signing up for and posting to mailing lists is upon us. I have banned the email address of the recent spam source and we are investigating methods to prevent these kinds of sign-ups/posts in the future. --Eric Eric Pettersen UCSF Computer Graphics Lab

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